This early article on “Pulse Fasting” says that Mag-10 is “spiked” with leucine, suggesting to me that it is not just casein hydrolysate, but that it has undergone a process similar to BCAA structured peptides that enhances the leucine content by cutting polypeptides at certain spots and filtering certain sizes.
Granted, the article was from 2010, and I didn’t start seeing people put empirical numbers on the leucine needed to produce a pulsing effect until early 2013, where studies showed that “as little as 2.5 grams of leucine could activate protein synthesis.” Later studies arrived at optimal levels ranging from 3.75 to 5.0, the later one playing it “on the safe side”.
Many of the early studies also used a standard dose of 25 grams of whey isolate, which would have just about 2.5 grams of leucine. Whey isolate should be somewhat slower to spike leucine levels than hydrolysate, which should also be slower than a di- and tri-peptide formulation (current research shows that the blood largely transports di- and tri-peptides, not needing to have them broken down to single amino acids, and di- and tri-peptides actually absorb even faster than individual amino acids). The fast absorption could get the leucine level up faster allowing a smaller dose to work, which would be great because it would allow more frequent pulsing. Here
you can see that 25 grams of mere whey isolate maximizes total amino acid concentration in the blood at 60 minutes with levels about 3/4 of the way to baseline by 3 hours. (Other research shows that amino acids from a normal meal typically clear completely in 4 hours-protein mildly raises insulin levels for 4 hours). Mag-10 should raise the levels faster though would not likely be faster than mono- and di-saccharides which peak in the blood at about 30 minutes. More recent work has strongly suggested that it is the leucine levels, and not the total amino acid levels that turn on protein synthesis, so plausibly, someone could consume low leucine protein throughout the day to prevent catabolism, but still pulse leucine levels over the levels of total amino acids with a high leucine source. Anyway, as of this time, a nutritional protocol should only be called “pulsing” (a term that now has a specific meaning in published research) if it provides at least 2.5 grams of leucine-the minimum amount shown in literature to produce a pulsing effect-or if research demonstrates a leucine spike on a lower dose with a faster absorbing product like Mag-10.